Optical photothermal infrared spectroscopy and discrete wavenumber imaging for high content screening of single cells
Tanveer Ahmed Shaik, Anuradha Ramoji, Nils Milis, Jürgen Popp, Christoph Krafft
Abstract
to visualize the nucleic acid, protein, and lipid distribution, respectively. Variations in subcellular features and in the lipid-to-protein and nucleic acid-to-protein ratios were identified that were consistent with biomolecular information in LDs. In conclusion, O-PTIR can provide high-quality spectra and images with submicron resolution of single cells in aqueous buffers that offer prospects in high-content screening applications.
Topics & Concepts
SpectroscopyHeLaChemistryAnalytical Chemistry (journal)Hyperspectral imagingNuclear magnetic resonancePhysicsChromatographyCellGeologyRemote sensingBiochemistryQuantum mechanicsSpectroscopy Techniques in Biomedical and Chemical ResearchSpectroscopy and Chemometric AnalysesThermography and Photoacoustic Techniques