Role of Mfd and GreA in Bacillus subtilis Base Excision Repair-Dependent Stationary-Phase Mutagenesis
Hilda C. Leyva-Sánchez, Norberto Villegas-Negrete, Karen Abundiz-Yañez, Ronald E. Yasbin, Eduardo A. Robleto, Mario Pedraza‐Reyes
Abstract
In this paper, we report that spontaneous genetic lesions of an oxidative nature in growing and nutritionally stressed B. subtilis strain YB955 ( hisC952 metB5 leuC427 ) cells drive Mfd- and GreA-dependent repair transactions. However, whereas Mfd and GreA elicit faithful repair events during growth to maintain genome fidelity, under starving conditions, both factors promote error-prone repair to produce genetic diversity, allowing B. subtilis to escape from growth-limiting conditions.
Topics & Concepts
BiologyBacillus subtilisMutagenesisLimitingGeneticsStrain (injury)MutationGeneBacteriaMechanical engineeringAnatomyEngineeringBacterial Genetics and BiotechnologyCRISPR and Genetic EngineeringDNA Repair Mechanisms