HPLC‐Based Automated Synthesis and Purification of Carbohydrates
Gustavo A. Kashiwagi, Laura Petrosilli, Samira Escopy, Luigi Lay, Keith J. Stine, C. De Meo, Alexei V. Demchenko
Abstract
Reported herein is a new HPLC-based automated synthesizer (HPLC-A) capable of a temperature-controlled synthesis and purification of carbohydrates. The developed platform allows to perform various protecting group manipulations as well as the synthesis of O- and N-glycosides. A fully automated synthesis and purification was showcased in application to different carbohydrate derivatives including glycosides, oligosaccharides, glycopeptides, glycolipids, and nucleosides.
Topics & Concepts
High-performance liquid chromatographyGlycosideChemistryGlycopeptideChromatographyCarbohydrateGlycolipidBiochemistryOrganic chemistryAntibioticsCarbohydrate Chemistry and SynthesisGlycosylation and Glycoproteins ResearchMicrobial Metabolites in Food Biotechnology