pSNAP: Proteome-wide analysis of elongating nascent polypeptide chains
Junki Uchiyama, Rohini Roy, Dan Ohtan Wang, Kazuya Morikawa, Yuka Kawahara, Mio Iwasaki, Chiaki Yoshino, Yuichiro Mishima, Yasushi Ishihama, Koshi Imami
Abstract
NPCs that characteristically exhibit protein N-terminus-biased positions. We applied pSNAP to evaluate the effect of silmitasertib, a potential molecular therapy for cancer, and revealed acute translational repression through casein kinase II and mTOR pathways. We also characterized modifications on NPCs and demonstrated that the combination of different types of modifications, such as acetylation and phosphorylation in the N-terminal region of histone H1.5, can modulate interactions with ribosome-associated factors. Thus, pSNAP provides a framework for dissecting co-translational regulations on a proteome-wide scale.