Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging
Chao Ren, Senyue Hao, Fei Wang, Abigail Matt, Marcello Magri Amaral, Daniel Yang, Leyao Wang, Chao Zhou
Abstract
Dynamic contrast optical coherence tomography (DyC-OCT), an emerging imaging method, utilizes fluctuation patterns in OCT signals to enhance contrast, thereby enabling non-invasive label-free volumetric live cell imaging. In this mini review, we explain the core concepts behind DyC-OCT image formation and its system configurations, serving as practical guidance for future DyC-OCT users. Subsequently, we explore its applications in delivering high-quality, contrast-enhanced images of cellular morphology, as well as in monitoring changes in cellular activity/viability assay experiments.
Topics & Concepts
Optical coherence tomographyContrast (vision)Computer scienceHigh contrastOptical imagingTomographyBiomedical engineeringComputer visionOpticsMedicinePhysicsOptical Coherence Tomography ApplicationsAdvanced Fluorescence Microscopy TechniquesCell Image Analysis Techniques