Establishment of a Novel Detection Platform for Clostridioides difficile Toxin Genes Based on Orthogonal CRISPR
Tong Jiang, Xinyi Hu, Jilu Shen
Abstract
C. difficile is currently the primary causative agent of hospital-acquired antibiotic-induced diarrhea, and timely and accurate diagnosis is crucial for hospital-acquired infection control and epidemiological investigation. Here, a new method for the identification of C. difficile was developed based on the recently popular CRISPR technology, and an orthogonal CRISPR dual system was utilized for the simultaneous detection of toxin genes A and B. It also uses a currently rare CRISPR dual-target lateral flow strip with powerful color-changing capabilities, which is appropriate for point-of-care testing (POCT).
Topics & Concepts
CRISPRClostridioidesClostridium difficileDiarrheaClostridium difficile toxin AClostridium difficile toxin BToxinGeneComputational biologyBiologyMicrobiologyAntibioticsMedicineGeneticsInternal medicineClostridium difficile and Clostridium perfringens researchCRISPR and Genetic EngineeringViral gastroenteritis research and epidemiology