hiPSC-CM electrophysiology: impact of temporal changes and study parameters on experimental reproducibility
Devon Guerrelli, Jenna Pressman, Shatha Salameh, Nikki Gillum Posnack
Abstract
electrophysiology measurements are impacted by deviations in experimental techniques including electrical stimulation protocols, equilibration time, well-to-well variability, and length of hiPSC-CM culture. Furthermore, our results indicate that hiPSC-CM drug responsiveness changes within the first 2 wk following defrost.
Topics & Concepts
ReproducibilityProarrhythmiaElectrophysiologyInduced pluripotent stem cellBiomedical engineeringMultielectrode arrayCardiac electrophysiologyCardiologyMedicineChemistryInternal medicinePharmacologyMicroelectrodeQT intervalEmbryonic stem cellGeneElectrodePhysical chemistryChromatographyBiochemistryNeuroscience and Neural Engineering3D Printing in Biomedical ResearchPluripotent Stem Cells Research