Real-Time and Spatial Electroanalysis of Biomolecules in One Living Cell Using Liquid-Phase Modified Nanopipette
Kang Liu, Rujia Liu, Dengchao Wang, Rongrong Pan, Hong‐Yuan Chen, Dechen Jiang
Abstract
Open AccessCCS ChemistryCOMMUNICATIONS10 Jan 2023Real-time and Spatial Electroanalysis of Biomolecules in one Living Cell using Liquid-phase Modified Nanopipette Kang Liu, Rujia Liu, Dengchao Wang, Rongrong Pan, Hong-Yuan Chen and Dechen Jiang Kang Liu Google Scholar More articles by this author , Rujia Liu Google Scholar More articles by this author , Dengchao Wang Google Scholar More articles by this author , Rongrong Pan Google Scholar More articles by this author , Hong-Yuan Chen Google Scholar More articles by this author and Dechen Jiang Google Scholar More articles by this author https://doi.org/10.31635/ccschem.023.202202397 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareFacebookTwitterLinked InEmail The well-developed solid-phase modified strategy at the electrode has enabled the preparation of biosensors for the detection of multiple analytes, even in single living cell. However, limited assay elements could be modified at the solid surface, restricting the analysis types of molecule and the detection sensitivity. Here, a novel liquid-phase modified strategy at the tip of nanopipette is designed to realize real-time and local analysis of biomolecules inside the cell that are hardly detected using solid-phase modified nanoelectrodes. This design utilizes the nanotip structure at a platinized carbon open nanopipette to stably retain a nano-droplet, which contains the required reagents with high reactivity for the assay of the target analyte. The generated hydrogen peroxide is electrochemically quantified at the Pt layer to realize the real-time measurement in a living cell with a spatial resolution of 70 nm. Taking the advantages of highly spatial and real-time detection, uneven distribution of sphingomyelinase (SMase) in the living CT26 cell is revealed unprecedentedly exhibiting the significance in the establishment of liquid-phase modified nanopipette. The new modification strategy will bring a new direction for the sensor design, and consequently, push the development of biosensors in the chemistry and biological study. Download figure Download PowerPoint Previous articleNext article FiguresReferencesRelatedDetails Issue AssignmentNot Yet Assigned Copyright & Permissions© 2023 Chinese Chemical Society Downloaded 0 times PDF downloadLoading ...