“On–off–on” pyrene‐based fluorescent chemosensor for the selective recognition of Cu<sup>2+</sup> and S<sup>2−</sup> ions and its utilization in live cell imaging
Rajasekaran Dhivya, Kavitha Venkatachalam, Viswanathamurthi Periasamy
Abstract
A new “on–off–on” fluorescence chemosensor (DPD) was designed, synthesized, and characterized based on pyrene and benzothiozole hydrazide. The probe DPD shows high affinity towards Cu 2+ ions and the DPD‐Cu 2+ ensemble shows high sensitivity towards S 2− ions through the displacement method in the presence of other interfering ions. The detection limits of DPD for Cu 2+ and S 2− ions were found to be 0.73 and 0.87 nM, respectively. Furthermore, the receptor was effectively applied to recognize Cu 2+ and S 2− ions in real samples and live A549 cells through imaging studies.
Topics & Concepts
ChemistryPyreneIonFluorescenceHydrazideLive cell imagingFluorescence-lifetime imaging microscopyAnalytical Chemistry (journal)PhotochemistryCellChromatographyOrganic chemistryBiochemistryPhysicsQuantum mechanicsMolecular Sensors and Ion DetectionLuminescence and Fluorescent MaterialsSulfur Compounds in Biology