Persister cells formation and expression of type II Toxin-Antitoxin system genes in Brucella melitensis (16M) and Brucella abortus (B19)
Fatemeh Amraei, Negar Narimisa, Behroz Sadeghi, Vahid Lohrasbi, Faramarz Masjedian Jazi
Abstract
BACKGROUND & OBJECTIVE: , cogT) expression. METHODS: strains treated with a lethal dose of gentamicin and ampicillin and to determine the number of surviving cells, bacterial colonies were counted at different time intervals. The role of TA systems in persister cell formation was then determined by toxin expression levels using qRT- PCR method. RESULTS: Our results showed the viability of persister cells after 7 h. The results of relative qRT- PCR showed higher levels of toxin gene expression due to stress conditions, suggesting the possible role of TA systems in persister cells formation and antibiotics tolerance. CONCLUSION: strains might help us to identify the precise mechanisms leading to persister cells formation.