Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance
Carolina Simão Albano, Anderson Moreira Gomes, Géorgia da Silva Feltran, Célio Júnior da Costa Fernandes, Luciana D. Trino, Willian Fernando Zambuzzi, Paulo Noronha Lisboa‐Filho
Abstract
BACKGROUND: Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces. METHODS: The biofunctionalization of titanium surfaces was characterized using classical physicochemical approaches and later used to challenge pre-osteoblast cells up to 24 h. Then their viability and molecular behavior were investigated using mitochondrial dehydrogenase activity and RTq-PCR technologies, respectively. Potential stimulus of extracellular remodeling was also investigated by zymography. RESULTS: ). CONCLUSION: analyses to better consider these surfaces before clinical trials within the biomedical field.