Rox8 promotes microRNA-dependent <i>yki</i> messenger RNA decay
Xiaowei Guo, Yihao Sun, Taha Azad, Helena J. Janse van Rensburg, Jingjing Luo, Shuai Yang, Peng Liu, Zhongwei Lv, Meixiao Zhan, Ligong Lu, Yingqun Zhou, Xianjue Ma, Xiaoping Zhang, Xiaolong Yang, Lei Xue
Abstract
Significance Dysregulation of the evolutionarily conserved Hippo pathway has been implicated in multiple diseases including cancer. Here we identified the RNA-binding protein (RBP) Rox8 as a regulator of Hippo signaling-mediated tumorigenesis. Rox8 not only directly binds to the 3′ UTR of yki mRNA, but also interacts with miR-8 to recruit miRNA-loaded RISC to degrade yki mRNA and therefore impedes Yki-induced tissue growth. We further revealed that TIAR, the human ortholog of Rox8, has retained a conserved regulatory function in yki / YAP mRNA stability. Our work uncovers a collaborative action of RBP and miRNA in regulating Hippo signaling.