Isolation of functional lysosomes from skeletal muscle
Thulasi Mahendran, Anastasiya Kuznyetsova, Neushaw Moradi, David A. Hood
Abstract
Lysosomes within skeletal muscle function to degrade dysfunctional debris and initiate retrograde signaling pathways. We developed a method to isolate purified lysosomal fractions using small portion of skeletal muscle, eliminating the need for density gradients or lysosome-modifying agents, ensuring high lysosomal purity without compromising structure or function. By enabling functional analysis via acid phosphatase, cathepsin-B activity, and calcium release, this approach offers a powerful tool to study lysosomal roles in muscle physiology, disease, and exercise.
Topics & Concepts
Skeletal muscleLysosomeCell biologyOrganelleBiologyBiochemistryMitochondrionAutophagyFunction (biology)MyocyteEnzymeChemistryCathepsinAdenosine triphosphateCatabolismATP hydrolysisCell physiologyProtein degradationHomeostasisNucleic acidAutophagy in Disease and TherapyCalcium signaling and nucleotide metabolismMitochondrial Function and Pathology