Gelatine–collagen photo-crosslinkable 3D matrixes for skin regeneration
Gauthier Menassol, Boudewijn van der Sanden, Laetitia Gredy, Capucine Arnol, Thibaut Divoux, Donald Martin, Olivier Stéphan
Abstract
using two-photon microscopy. For both types of matrixes, the adherence and proliferation of fibroblasts (3T3-NIH) were optimal for stiff structures with a Young's modulus of 191 ± 35 kPa compared to softer matrixes of 37 ± 12 kPa. Fibroblast showed complete confluence on Day 14 after seeding on these matrixes, which may create the granulation tissue composed of fibronectin, collagen, and various proteoglycans in the future dermis before repair of the epidermis and disintegrating of their host matrix. For the monitoring of this repair, gelatine-collagen matrixes can easily incorporate bio-optical sensors for the simultaneous monitoring of inflammation processes and wound healing in time.