H <sub>2</sub> O <sub>2</sub> -Driven Hydroxylation of Steroids Catalyzed by Cytochrome P450 CYP105D18: Exploration of the Substrate Access Channel
Bashu Dev Pardhe, Kyoung Pyo Kwon, Jong Kook Park, Jun Hyuck Lee, Tae‐Jin Oh
Abstract
-mediated hydroxylation of steroids by CYP105D18, which also overcomes the use of expensive cofactors. Further, exploring the substrate access channel and modifying the bulky amino acid F184A increase substrate conversion while modifying the substrate recognizing amino acid S63 markedly decreases product formation. Exploring the substrate access channel and the rational design of CYP105D18 can improve the substrate conversion, which facilitates the engineering of P450s for industrial application.
Topics & Concepts
HydroxylationSubstrate (aquarium)CatalysisChannel (broadcasting)Cytochrome P450ChemistryCytochromeStereochemistryCombinatorial chemistryComputer scienceEnzymeBiochemistryBiologyTelecommunicationsEcologyPharmacogenetics and Drug MetabolismSteroid Chemistry and BiochemistryHormonal Regulation and Hypertension