Combining a hybrid chip and tube microfluidic system with fluorescent molecularly imprinted polymer (MIP) core–shell particles for the derivatisation, extraction, and detection of peptides with N-terminating phosphorylated tyrosine
Samual Charles Burnage, Jérémy Bell, Wei Wan, Evgeniia Kislenko, Knut Rurack
Abstract
A microfluidic ratiometric detection scheme using silica nanoparticles coated with a “light-up” fluorescent molecularly imprinted polymer allows selective rapid assaying of pY -terminating peptides after in-tube/on-chip derivatisation and extraction.
Topics & Concepts
Molecularly imprinted polymerMicrofluidicsFluorescenceExtraction (chemistry)PolymerChipChromatographyMicrofluidic chipMaterials scienceNanotechnologyChemistryOrganic chemistrySelectivityEngineeringComposite materialPhysicsElectrical engineeringQuantum mechanicsCatalysisAnalytical chemistry methods developmentBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniques