Genomic-transcriptomic analysis of Staphylococcus aureus biofilm formation under sub-MIC antibiotic exposure
Zhenbo Xu, Yaqin Li, Liang Xue, Aijuan Xu, Guangchao Yu, Thanapop Soteyome, Lei Yuan, Xuejie Li, Junyan Liu
Abstract
Antibiotics are widely used in animal husbandry to ensure the health of livestock, leading to the exposure of microorganisms to accumulated sub-lethal concentrations (sub-MICs) of antibiotics in meats. This study aimed to investigate the effects and mechanisms of sub-MICs of commonly used antibiotics on the biofilm formation of a S. aureus strain Guangzhou-SAU071 which displays weak biofilm formation despite harboring biofilm-associated genes. CV and MTS assays were used to determine biofilm biomass and cell viability, respectively. Dual-omics sequencing combining genomics and transcriptomics was used to study the global expression changes. Expression of biofilm and two-component system associated genes was further verified by RT-qPCR. Biofilm formation of Guangzhou-SAU071 was enhanced under sub-MIC of ciprofloxacin (2 μg/mL) and streptomycin (128 μg/mL). Nearly half of the genes associated with biofilm formation, cell wall anchoring, and two-component systems exhibited significant differential expression under sub-MIC of ciprofloxacin and streptomycin. As concluded, sub-MIC of ciprofloxacin and streptomycin enhanced biofilm formation of S. aureus, possibly due to its regulation on biofilm and two-component system associated genes.