Identification and genotyping of a new subtype of bovine viral diarrhea virus 1 isolated from cattle with diarrhea
Bin Tian, Dongjie Cai, Weiqiang Li, Qinglong Bu, Mingshu Wang, Gang Ye, Jianzhu Liu, Ya Wang, Liping Gou, Jun Yi, Zhicai Zuo
Abstract
In 2019, diarrhea cases occurred on cattle farms in Qionglai and Guang'an, Sichuan Province. Two out of 20 (10%) serum and nasal swab samples were positive when tested using a bovine viral diarrhea virus (BVDV) antigen-capture ELISA kit. Two non-cytopathic strains of BVDV were isolated and named QL1903 and GA190608, respectively. The nucleotide sequences of the genomes of the two isolates were 89.52% identical. Phylogenetic analysis based on the 5'-UTR sequence revealed that the BVDV isolate QL1903 belonged to BVDV subtype 1b, whereas isolate GA190608 clustered with strains HN1814, EN-19, and BJ09_26 in a separate branch, which has tentatively been classified as a new genetic subtype, "1v".