Effects of Local Sequence, Reaction Conditions, and Various Additives on the Formation and Stability of Interstrand Cross-Links Derived from the Reaction of an Abasic Site with an Adenine Residue in Duplex DNA
Saosan Binth Md. Amin, Tanhaul Islam, Nathan E. Price, Amanda Wallace, Xu Guo, Anuoluwapo Gomina, Marjan Heidari, Kevin M. Johnson, Calvin D. Lewis, Zhiyu Yang, Kent S. Gates
Abstract
CN, by the intercalator ethidium bromide, and by the minor groove-binding agent netropsin. ICL formation was inhibited to some extent in bicarbonate and Tris buffers. The dA-AP ICL showed substantial inherent stability under a variety of conditions and was not a substrate for AP-processing enzymes APE1 or Endo IV. Finally, we characterized cross-link formation in a small (11 bp) stem-loop (hairpin) structure and in DNA-RNA hybrid duplexes.
Topics & Concepts
ChemistryDuplex (building)AP siteStereochemistryDNAEthidium bromideDNA glycosylaseBiochemistryDNA damageDNA and Nucleic Acid ChemistryDNA Repair MechanismsAcute Lymphoblastic Leukemia research