A roadmap for metagenomic enzyme discovery
Serina L. Robinson, Jörn Piel, Shinichi Sunagawa
Abstract
methods for enzyme discovery including phylogenetics, sequence similarity networks, genomic context, 3D structure-based approaches, and machine learning techniques. We also discuss various experimental strategies to test computational predictions including heterologous expression and screening. Finally, we provide an outlook for future directions in the field with an emphasis on meta-omics, single-cell genomics, cell-free expression systems, and sequence-independent methods.
Topics & Concepts
MetagenomicsComputational biologyDrug discoveryData scienceBiologyComputer scienceBioinformaticsBiochemistryGeneGenomics and Phylogenetic StudiesMicrobial Metabolic Engineering and BioproductionMicrobial Natural Products and Biosynthesis