Bioprinting of Cartilage with Bioink Based on High-Concentration Collagen and Chondrocytes
Е. Е. Бекетов, Елена Исаева, Nina D. Yakovleva, Grigory Demyashkin, Nadezhda V. Arguchinskaya, Anastas Kisel, Tatiana S. Lagoda, Egor P. Malakhov, Valentin I. Kharlov, E. O. Osidak, S.P. Domogatsky, С. А. Иванов, Petr Shegay, А. Д. Каприн
Abstract
cartilage formation. Extrusion-based bioprinting was used for the biofabrication. The printing parameters were tuned to obtain stable material flow. In vivo data proved the ability of the tested bioink to form a cartilage within five to six weeks after the subcutaneous scaffold implantation. Certain areas of cartilage formation were detected as early as in one week. The resulting cartilage tissue had a distinctive structure with groups of isogenic cells as well as a high content of glycosaminoglycans and type II collagen.