Two-stage tuberculosis diagnostics: combining centrifugal microfluidics to detect TB infection and Inh and Rif resistance at the point of care with subsequent antibiotic resistance profiling by targeted NGS
Judith Schlanderer, Harald Hoffmann, Jan Lüddecke, Andrey Golubov, Wolfgang Grasse, Elisabeth V. Kindler, Thomas A. Kohl, Matthias Merker, Christoph Metzger, Vanessa Mohr, Stefan Niemann, Claudia Pilloni, Sara Plesnik, Bijendra Raya, Bhawana Shresta, Christian Utpatel, Roland Zengerle, Markus Beutler, Nils Paust
Abstract
, a diagnostic sensitivity of 96%, and a diagnostic specificity of 100% for MTBC detection. Coupled tNGS successfully provided resistance profiles, demonstrated for samples from 17 patients. To the best of our knowledge, the presented combination of PoC qPCR with tNGS allows for the fastest comprehensive TB diagnostics comprising decentralized pathogen detection with subsequent resistance profiling in a facility specialized in tNGS.
Topics & Concepts
TuberculosisAntibiotic resistancePoint of careAntibioticsMedicineMicrofluidicsDrug resistanceProfiling (computer programming)MicrobiologyVirologyBiologyNanotechnologyMaterials sciencePathologyComputer scienceOperating systemBacterial Identification and Susceptibility TestingMicrofluidic and Capillary Electrophoresis ApplicationsBiosensors and Analytical Detection