Bioinspired Structure Regulation of Apyrase-Like Nanozyme with Intracellular-Generated H<sub>2</sub>O<sub>2</sub> for Tumor Catalytic Therapy
Yadong Zhe, Wang Zhang, Chaoyue Gu, Lu Sun, Fangdi Dong, Zhiqiang Zhao, Kai Li, Yuqing Lin
Abstract
Adenosine triphosphate (ATP) is the major resource of energy supply in tumor activity. Therefore, improving ATP consumption efficiencies is a promising approach for cancer therapy. Herein, inspired by the H 2 O 2 -involved structure regulation effect during the catalysis of natural protein enzymes, we developed an artificial H 2 O 2 -driven ATP catalysis-promoting system, the Ce-based metal–organic framework (Ce-MOF), for catalytic cancer therapy. In the presence of H 2 O 2, the hydrolysis ATP activity of Ce-MOF(H 2 O 2 ) was enhanced by around 1.6 times. Taking advantage of the endogenous H 2 O 2 in cancerous cells, catalytic hydrolysis for intracellular ATP of the Ce-MOF achieves the inhibition of cancerous cell growth, which involves damaged mitochondrial function and autophagy-associated cell death. Furthermore, in vivo studies suggest that the Ce-MOF has a good tumor inhibition effect. The artificial H 2 O 2 -driven ATP catalysis-promoting system not only demonstrates high catalytic ATP consumption efficiencies for cancer therapy but also highlights a bioinspired strategy to expedite nanozyme research in both design and applied sciences.