A dual-responsive ratiometric indicator designed for <i>in vivo</i> monitoring of oxidative stress and antioxidant capacity
Majun Yang, Weida Zhu, Yilin Lv, Bin Jiang, Chenxia Jiang, Xiaobo Zhou, Li Guo, Yuling Qin, Qi Wang, Ziwei Chen, Li Wu
Abstract
experiments demonstrated that the levels of the two cardiac biomarkers increase progressively with the development of myocardial infarction, eventually reaching a steady state after 7 days when the damaged cells in the infarcted region become depleted. Moreover, during 14 continuous days of exposure to trichloroethylene, the two biomarkers in liver tissue exhibited a sustained increase, indicating a significant enhancement in intracellular oxidative stress and antioxidant capacity attributed to the mouse liver's robust metabolic capacity. The aforementioned studies underscore the efficacy of DQ1 as a valuable tool for scrutinizing redox states at both the single-cell and biological tissue levels. It presents significant potential for investigating the dynamic alternations in oxidative stress and antioxidant capacity within disease models as the disease progresses, thereby facilitating a more profound comprehension of these processes across various disease models.