Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering
Laurens Raes, Melissa Pille, Aranit Harizaj, Glenn Goetgeluk, Jelter Van Hoeck, Stephan Stremersch, Juan C. Fraire, Toon Brans, Olivier G. de Jong, Roel F. Maas-Bakker, Enrico Mastrobattista, Pieter Vader, Stefaan C. De Smedt, Bart Vandekerckhove, Koen Raemdonck, Kevin Braeckmans
Abstract
engineering of cell therapy products.
Topics & Concepts
TransfectionEx vivoIn vivoCell biologyNanotechnologyChemistryMaterials scienceBiologyBiotechnologyGeneBiochemistryCRISPR and Genetic EngineeringViral Infectious Diseases and Gene Expression in InsectsVirus-based gene therapy research