Temporal dynamics of the multi-omic response to endurance exercise training
MoTrPAC Study Group, Primary authors, Lead Analysts, David Amar, Nicole R. Gay, Pierre M. Jean Beltran, Lead Data Generators, Dam Bae, Surendra Dasari, Courtney Dennis, Charles R. Evans, David A. Gaul, Olga Ilkayeva, Anna Ivanova, Maureen Kachman, Hasmik Keshishian, Ian R. Lanza, Ana C. Lira, Michael J. Muehlbauer, Venugopalan D. Nair, Paul Piehowski, Jessica L. Rooney, Kevin S. Smith, Cynthia L. Stowe, Bingqing Zhao, Analysts, Natalie M. Clark, David Jimenez‐Morales, Maléne E. Lindholm, Gina M. Many, James Sanford, Gregory R. Smith, Nikolai G. Vetr, Tiantian Zhang, José Juan Almagro Armenteros, Julián Ávila-Pacheco, Nasim Bararpour, Yongchao Ge, Zhenxin Hou, Shruti Marwaha, David M. Presby, Archana N. Raja, Evan Savage, Alec Steep, Yifei Sun, Si Wu, Jimmy Zhen, Animal Study Leadership, Sue C. Bodine, Karyn A. Esser, Laurie J. Goodyear, Simon Schenk, Manuscript Writing Group Leads, Manuscript Writing Group, Stephen B. Montgomery, Facundo M. Fernández, Stuart C. Sealfon, M Snyder, Senior Leadership, Joshua Adkins, Euan A. Ashley, Charles Burant, Steven A. Carr, Clary B. Clish, Gary Cutter, Robert E. Gerszten, William E. Kraus, Jun Z. Li, Michael E. Miller, K. Sreekumaran Nair, Christopher B. Newgard, Eric A. Ortlund, Weijun Qian, Russell P. Tracy, Martin J. Walsh, Matthew T. Wheeler, Co-corresponding Authors, MoTrPAC Study Group, Karen Dalton, Trevor Hastie, Steven G. Hershman, Mihir Samdarshi, Christopher Teng, Rob Tibshirani, Biospecimens Repository, Elaine Cornell, Nicole Gagne, Sandy May, Brian Bouverat, Christiaan Leeuwenburgh, Ching-ju Lu, Marco Pahor, Fang‐Chi Hsu, Scott Rushing, Michael P. Walkup, Exercise Intervention Core, Barbara J. Nicklas, W. Jack Rejeski, NIH, John P. Williams
Abstract
profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome and immunome in whole blood, plasma and 18 solid tissues in male and female Rattus norvegicus over eight weeks of endurance exercise training. The resulting data compendium encompasses 9,466 assays across 19 tissues, 25 molecular platforms and 4 training time points. Thousands of shared and tissue-specific molecular alterations were identified, with sex differences found in multiple tissues. Temporal multi-omic and multi-tissue analyses revealed expansive biological insights into the adaptive responses to endurance training, including widespread regulation of immune, metabolic, stress response and mitochondrial pathways. Many changes were relevant to human health, including non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular health and tissue injury and recovery. The data and analyses presented in this study will serve as valuable resources for understanding and exploring the multi-tissue molecular effects of endurance training and are provided in a public repository ( https://motrpac-data.org/ ).