Genomic profiling of native R loops with a DNA-RNA hybrid recognition sensor
Wang Kang, Honghong Wang, Conghui Li, Zhinang Yin, R. Xiao, Qiuzi Li, Ying Xiang, Wen Wang, Jian Huang, Liang Chen, Pingping Fang, Kaiwei Liang
Abstract
-transferase-hexahistidine-2× hybrid-binding domain), an artificial DNA-RNA hybrid sensor that specifically recognizes the DNA-RNA hybrids. We demonstrate that the R loop CUT&Tag is sensitive, reproducible, and convenient for native R loop mapping with high resolution, and find that the capture strategies, instead of the specificity of sensors, largely contribute to the disparities among different methods. Together, we provide an independent strategy for genomic profiling of native R loops and help resolve discrepancies among multiple R loop mapping methods.
Topics & Concepts
Computational biologyRNADNABiologyLoop (graph theory)Profiling (computer programming)GeneticsComputer scienceGeneMathematicsOperating systemCombinatoricsGenomics and Chromatin DynamicsAdvanced biosensing and bioanalysis techniquesRNA modifications and cancer