Minimal Change Disease Is Associated With Endothelial Glycocalyx Degradation and Endothelial Activation
Colin Bauer, Federica Piani, Mindy Banks, Flor Á. Ordóñez, Carmen de Lucas Collantes, K. Oshima, Eric P. Schmidt, Igor Zakharevich, Alfons Segarra, Cristina Martínez, Carlos A. Roncal-Jiménez, Simon C. Satchell, Petter Bjornstad, M. Scott Lucia, Judith Blaine, Joshua M. Thurman, Richard J. Johnson, Gabriel Cara‐Fuentes
Abstract
Introduction: Minimal change disease (MCD) is considered a podocyte disorder triggered by unknown circulating factors. Here, we hypothesized that the endothelial cell (EC) is also involved in MCD. Methods: 5 per group) and performed Western blotting of thrombomodulin of cell lysates as surrogate marker of endothelial activation. Results: In circulation, median concentrations of all endothelial markers were higher in patients with active disease compared with controls and remained high in some patients during remission. In the MCD glomerulus, caveolin-1 expression was higher, in an endothelial-specific pattern, compared with controls. In cultured human GEnC, sera from children with MCD/SSNS in relapse increased thrombomodulin expression compared with control sera. Conclusion: Our data show that alterations involving the systemic and glomerular endothelium are nearly universal in patients with MCD and SSNS, and that GEnC can be directly activated by circulating factors present in the MCD/SSNS sera during relapse.