Synthesis, spectral characterization, lethal dose (LD50) and acute toxicity studies of 1,4-Bis(imidazolylazo)benzene (BIAB)
Hussein Ali Kadhim Kyhoiesh, Mohammed Kassim Al-Hussainawy, Azal S. Waheeb, Khalid J. Al-Adilee
Abstract
The preparation and spectral identification of new heterocyclic azo ligand 1,4-Bis(imidazolylazo)benzene (BIAB) was prepared by reacting a diazonium chloride salt solution of 1,4-diaminobenzene with imidazole in alkaline ethanolic solution. Differing spectral techniques have been used to study the structure of the azo dye ligand (BIAB) such as Elemental analysis (C.H.N), 1 H-NMR, Mass spectrum, UV-Vis, FT-IR, XRD, FE-SEM and thermal analysis (TGA-DTA). The pathogenic activities of the synthesized ligand (BIAB) was tested in vitro against the sensitive organisms Staphylococcus aureus (Gram-positive) and Escherichia coli (gram-negative) as antibacterial and Aspergillus Niger and Candida albicans as antifungal. The activity data show that the ligand (BIAB) higher antibacterial and slightly antifungus activity in comparison to the standard antibacterial ( Amoxicillin ) and antifungal ( cycloheximide ) drugs. The acute toxicity studies (LD 50 ) was calculated using by Miller and Tainter methods (Estimated Probity Units) for the calculation of LD 50 . In this study, different doses (600, 1000, 1300, 1800, 2500 and 3600 μg/ml) of the (BIAB) was administered orally to the different groups of mice. The results exhibited high acute toxicity with LD 50 of 1020.23 mg/kg upon intraperitoneal administration in mice. The antioxidant properties of the ligand was examined using the DPPH radical scavenging technique. IC 50 was also determined at 224.17 μg/ml.