Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS
Momoko Kawana, Masatoshi Miyamoto, Yusuke Ohno, Akio Kihara
Abstract
) configuration. Genetic knockout of β-hydroxy acyl-CoA dehydratases in HAP1 cells increased β-hydroxy ceramide levels, suggesting that β-hydroxy acyl-CoA, an FA-elongation cycle intermediate in the ER, is a substrate for β-hydroxy ceramide synthesis. We anticipate that our methods and findings will help to elucidate the role of each ceramide class in skin barrier formation and in the pathogenesis of skin disorders.
Topics & Concepts
CeramideStratum corneumSphingolipidSphingosineChemistryBiochemistryBarrier functionBiologyCell biologyGeneticsApoptosisReceptorAdvancements in Transdermal Drug DeliveryDermatology and Skin DiseasesSphingolipid Metabolism and Signaling