Bromelia karatas and Bromelia pinguin: sources of plant proteases used for obtaining antioxidant hydrolysates from chicken and fish by-products
Martha Guillermina Romero-Garay, Emmanuel Martínez‐Montaño, Adrián Hernández‐Mendoza, Belinda Vallejo‐Córdoba, Aarón F. González‐Córdova, Efigenia Montalvo‐González, María de Lourdes García‐Magaña
Abstract
Abstract In the present study, we evaluated new sources of plant proteases from fruits of Bromelia karatas (BK) and Bromelia pinguin (BP) to obtain antioxidant hydrolyzates/bioactive peptides (BPs) derived from chicken by-products (CH) and fish by-products (FH). The profile of the peptides was identified by reverse-phase high-resolution liquid chromatography (RP-HPLC) and the size weight distribution by molecular exclusion chromatography (SEC). The hydrolysates obtained with BK in both sources of by-products showed greater antioxidant capacity compared to those obtained with BP, presenting similar or higher values when compared to a commercial plant enzyme. The use of new sources of plant proteases allowed to obtain hydrolysates of hydrophilic character with a high percentage (> 50%) of peptides with molecular weights < 17.5 kDa from chicken and fish by-products. Therefore, based on the results obtained in antioxidant capacity it is possible to consider the hydrolysates as potential ingredients, food additives, and pharmaceutical products.