Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells
Chia‐Hung Christine Hsiao, Andrew J. Wiemer
Abstract
Vγ9Vδ2 T cells are non-canonical T cells that use their T cell receptor to detect phosphoantigens bound to the internal domain of the HMBPP receptor (butyrophilin 3/2A1 complex). This protocol describes the expansion and purification of human effector Vγ9Vδ2 T cells from human buffy coat and describes how to assess their activation by antigen-containing target cells. While specifically focused on cytokine production, this protocol can be readily adapted to evaluate other effector functions of activated Vγ9Vδ2 T cells. For complete details on the use and execution of this protocol, please refer to Hsiao et al. (2022) and Hsiao and Wiemer (2018).
Topics & Concepts
EffectorCell biologyT-cell receptorIL-2 receptorStreptamerBuffy coatT cellAntigenBiologyMolecular biologyChemistryComputer scienceImmunologyImmune systemT-cell and B-cell ImmunologyImmune Cell Function and InteractionImmunotherapy and Immune Responses