Using AnnoTree to Get More Assignments, Faster, in DIAMOND+MEGAN Microbiome Analysis
Anupam Gautam, Hendrik Felderhoff, Caner Bağcı, Daniel H. Huson
Abstract
The NCBI-nr database is not explicitly designed for the purpose of microbiome analysis, and its increasing size makes its unwieldy and computationally expensive for this purpose. The AnnoTree protein database is only one-quarter the size of the full NCBI-nr database and is explicitly designed for metagenomic analysis, so it should be supported by alignment-based pipelines.
Topics & Concepts
MicrobiomeDiamondComputational biologyComputer scienceData scienceBiologyChemistryGeneticsOrganic chemistryGenomics and Phylogenetic StudiesMicrobial Community Ecology and PhysiologyGut microbiota and health