An activator-induced quencher-detachment-based turn-on probe with a cationic substrate moiety for acetylcholinesterase
Masahiro Oe, Koji Miki, Akito Masuda, Kohei Nogita, Kouichi Ohe
Abstract
We report a choline ester-grafted turn-on fluorescence probe to detect acetylcholinesterase (AChE) in living cells. The AChE-mediated hydrolysis of the choline ester moiety producing carboxylate initiates the activation of the Cy5 fluorophore quenched by an intramolecular nucleophilic mercapto group. The probe has the advantages of high AChE affinity and low cytotoxicity.
Topics & Concepts
MoietyChemistryAcetylcholinesteraseFluorophoreCationic polymerizationNucleophileCarboxylateCytotoxicityIntramolecular forceSubstrate (aquarium)HydrolysisPhotochemistryFluorescenceStereochemistryCombinatorial chemistryEnzymeOrganic chemistryBiochemistryCatalysisIn vitroGeologyPhysicsOceanographyQuantum mechanicsCholinesterase and Neurodegenerative DiseasesPesticide Exposure and ToxicityNicotinic Acetylcholine Receptors Study