Single-molecule fluorescence detection of a tricyclic nucleoside analogue
George N. Samaan, Mckenzie K. Wyllie, Julian M. Cizmic, Lisa-Maria Needham, David Nobis, Katrina Ngo, Susan Andersen, Steven W. Magennis, Steven F. Lee, Byron W. Purse
Abstract
= 0.50-0.53 when base paired with adenine in duplex DNA oligonucleotides. Single-molecule fluorescence measurements of ABN using both one-photon and two-photon excitation demonstrate its excellent photostability and indicate that the nucleoside is present to > 95% in a bright state with count rates of at least 15 kHz per molecule. This new fluorescent nucleobase analogue, which, in duplex DNA, is the brightest and most red-shifted known, is the first to offer robust and accessible single-molecule fluorescence detection capabilities.
Topics & Concepts
FluorescenceChemistryMoleculeNucleobaseTricyclicNucleosideDNAOligonucleotidePyrimidineStereochemistryPhotochemistryOrganic chemistryBiochemistryQuantum mechanicsPhysicsAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryPhotochemistry and Electron Transfer Studies