Probing conformational hotspots for the recognition and intervention of protein complexes by lysine reactivity profiling
Zheyi Liu, Wenxiang Zhang, Binwen Sun, Yaolu Ma, Min He, Yuanjiang Pan, Fangjun Wang
Abstract
A mass spectrometry-based two-step isotope labeling-lysine reactivity profiling strategy is developed to probe the molecular details of protein–protein interactions and evaluate the conformational interventions by small-molecule active compounds.
Topics & Concepts
LysineChemistryProfiling (computer programming)Mass spectrometryReactivity (psychology)MoleculeComputational biologyCombinatorial chemistryComputational chemistryBiophysicsBiochemistryBiologyComputer scienceOrganic chemistryMedicineChromatographyAmino acidOperating systemAlternative medicinePathologyBiotin and Related StudiesComputational Drug Discovery MethodsProtein Structure and Dynamics