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Diverse routes to mitophagy governed by ubiquitylation and mitochondrial import

Michael J. Clague, Sylvie Urbé

2025Trends in Cell Biology33 citationsDOIOpen Access PDF

Abstract

<h2>Abstract</h2> The selective removal of mitochondria by mitophagy proceeds via multiple mechanisms and is essential for human well-being. The PINK1/Parkin and NIX/BNIP3 pathways are strongly linked to mitochondrial dysfunction and hypoxia, respectively. Both are regulated by ubiquitylation and mitochondrial import. Recent studies have elucidated how the ubiquitin kinase PINK1 acts as a sensor of mitochondrial import stress through stable interaction with a mitochondrial import supercomplex. The stability of BNIP3 and NIX is regulated by the SCF<sup>FBXL4</sup> ubiquitin ligase complex. Substrate recognition requires an adaptor molecule, PPTC7, whose availability is limited by mitochondrial import. Unravelling the functional implications of each mode of mitophagy remains a critical challenge. We propose that mitochondrial import stress prompts a switch between these two pathways.

Topics & Concepts

MitophagyPINK1ParkinUbiquitin ligaseBiologyCell biologyUbiquitinMitochondrionAutophagyBiochemistryGeneApoptosisMedicinePathologyDiseaseParkinson's diseaseAutophagy in Disease and TherapyMitochondrial Function and PathologyUbiquitin and proteasome pathways
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