Identification of a novel ene reductase from <i>Pichia angusta</i> with potential application in (<i>R</i>)-levodione production
Baoqi Zhang, Jiale Sun, Yanqiu Zheng, Xinlei Mao, Jinping Lin, Dongzhi Wei
Abstract
Asymmetric reduction of electronically activated alkenes by ene reductases (ERs) is an attractive approach for the production of enantiopure chiral products.
Topics & Concepts
Enantiopure drugBiocatalysisChemistryEnzyme kineticsCofactorEne reactionStereoselectivityStereochemistryYield (engineering)ReductaseEnzymeEscherichia coliEnantioselective synthesisBiochemistryReaction mechanismActive siteCatalysisMaterials scienceMetallurgyGeneEnzyme Catalysis and ImmobilizationPharmacogenetics and Drug MetabolismSteroid Chemistry and Biochemistry