Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined media
Sylvain Perriot, Mathieu Canales, Amandine Mathias, Renaud Du Pasquier
Abstract
This protocol describes how to obtain human astrocytes from human-induced pluripotent stem cells (hiPSCs) in chemically defined media, without the use of fetal bovine serum (FBS). FBS eases the differentiation of astrocytes but also deeply alters their phenotype, as compared with their in vivo counterparts. Our protocol generates hiPSC-derived astrocytes displaying a phenotype and functions similar to human primary astrocytes, including adequate response to inflammation, neurotransmitter uptake, and trophic support to neurons. For complete details on the use and execution of this protocol, please refer to Perriot et al. (2018).
Topics & Concepts
Human Induced Pluripotent Stem CellsInduced pluripotent stem cellPhenotypeCell biologyAstrocyteBiologyCellular differentiationNeuroscienceStem cellChemistryEmbryonic stem cellBiochemistryCentral nervous systemGenePluripotent Stem Cells ResearchAnesthesia and Neurotoxicity ResearchNeuroinflammation and Neurodegeneration Mechanisms