Chemical composition and anti-inflammatory activity of essential oils from resin of Commiphora species
Worku Dinku, Su Bin Park, Jin Boo Jeong, Chuleui Jung, Aman Dekebo
Abstract
ABSTRACT. Essential oils (EOs) were prepared by the hydro-distillation technique from the resins of four Commiphora species and analyzed by GC-MS. Major constituents of EOs were a-copaene (22.71%), β-caryophyllene (28.03%) and β-caryophyllene oxide (13.89%) for C. sphaerocarpa; a-pinene (29.1%) for C. africana; hexadecane (14.1%) for C. habessinica and δ-cadinene (31.5%) for C. schimperi. We investigated the anti-inflammatory effects of EOs in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages by measuring nitric oxide (NO). The effect in mRNA or protein level after EO treatment were evaluated by RT-PCR and Western blot analysis, respectively. Among four Commiphora species, C. sphaerocarpa EO demonstrated a significant inhibition of LPS by 27.2±3.6% at 10 μg/mL and 62.3±5.2% at 20 μg/mL. C. sphaerocarpa EO inhibited LPS mediated iNOS over expression in both protein and mRNA level with dose dependent manner. It inhibited phosphorylation of ERK1/2, p38, ATF2. The enhanced anti-inflammatory activity of the EO of the plant was due to HO-1 expression by ROS dependent Nrf2 activation in RAW264.7 cells. These findings indicate C. sphaerocarpa EO inhibits the pro-inflammatory responses by inhibiting MAPK/ATF2, and triggering ROS/Nrf2/HO-1 signaling. Therefore, C. sphaerocarpa EO could have potential for useful therapeutic candidate preventing and treating inflammatory diseases. KEY WORDS: GC-MS, Anti-inflammatory, C. africana, C. habessinica, C. sphaerocarpa, C. schimperi Bull. Chem. Soc. Ethiop. 2022, 36(2), 399-415. DOI: https://dx.doi.org/10.4314/bcse.v36i2.13