Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
Jie Zhou, Meng Liang, Yu Lin, Hao Pang, Yutuo Wei, Ribo Huang, Liqin Du
Abstract
Resveratrol, an ingredient of traditional Chinese medicine, has beneficial effects on human health and huge potential for application in modern medicine. Polydatin is extracted from plants and then deglycosylated into resveratrol; enzymatic methods are preferred for this reaction. In this study, a β-D-glucosidase from Sphingomonas showed high efficiency in transforming polydatin into resveratrol and was tolerant toward organic solvents. Applying this enzyme in a biphasic transformation system resulted in 95.3% conversion of 20% concentration crude polydatin to resveratrol in 4 h. We thus report a new method for high-efficiency, clean production of resveratrol.
Topics & Concepts
ResveratrolChemistryIngredientTransformation (genetics)EnzymeHuman healthBiochemistryPharmacologyFood scienceMedicineEnvironmental healthGeneSirtuins and Resveratrol in MedicineCRISPR and Genetic EngineeringPlant tissue culture and regeneration