Bioengineered TiO<sub>2</sub> Nanoparticles Using <i>Andrographis alata</i> (Vahl) Nees Leaf Extract and Their Antibacterial and Anticancer Activities
Aswini Rangayasami, Karthik Kannan, Murugesan Subban
Abstract
Abstract Breast cancer is one of the major causes of increased deaths and it is the focus of researchers across the world. Bacteria are harmful to create human diseases like cholera, jaundice. It is needful to control the growth of harmful bacteria. Andrographis alata is rarely presented, widely used in tribal people, and it is also useful to many diseases. These studies are focused on the control the growth of (Gram‐positive (G +ve ) and negative (G −ve )) bacteria, and evaluate the anticancer activity against the MCF‐7 cell line using TiO 2 nanoparticles (NPs). TiO 2 NPs is prepared via A. alata leaf extract. The synthesized TiO 2 NPs are characterized by Fourier‐transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), and transmission electron microscope (TEM). Antibacterial activity is carried out by disc diffusion method. The anti‐breast cancer activity (MCF‐7 cell line) is determined via MTT assay. The antibacterial activities of TiO 2 NPs are tested against the G +ve ( Streptococcus pneumonia and Bacillus cereus ) and G −ve ( Pseudomonas aeruginosa and Escherichia coli ). The greatest zone of inhibition is observed in B. cereus (15 mm) and E. coli (18 mm) at 50 µL. In vitro anticancer efficiency (IC 50 ) of synthesized TiO 2 NPs shows good anticancer activity against the MCF‐7 cancer cell line. The prepared NPs show potent antibacterial (foodborne pathogens) and anticancer (MCF‐7) activities, so TiO 2 NPs will be useful in biomedical applications.