Optimization of exopolysaccharide produced by <i>L. kefiranofaciens</i> ZW3 using response surface Methodology
Zaheer Ahmed, Tahir Mehmood, Ifra Ferheen, Abdul Wasim Noori, Majid Almansouri, Muhammad Waseem
Abstract
The primary objective of this study was to optimize the production of exopolysaccharide (EPS) produced by L. kefiranofaciens ZW3 through the utilization of response surface methodology (RSM). Central Composite Design (CCD) was used along with three variables to study the response variable. The effects of lactose (0.16–1.84%), tryptophan (0.16–0.84%), and pH (5.06–7.24) on EPS production were studied. RSM analysis revealed that coefficient of determination (0.9907) was good for the second-order polynomial model. The optimum values of explanatory variables were 0.36% lactose, 0.23% tryptophan, and pH value of 5.19. Additional experiments were performed to confirm the adequacy of this model. The predicted value of EPS at optimized level was 1609 mg/L, while its experimental value at optimized level was 1602 ± 3 mg/L. This study could help to produce higher amount of EPS from L. kefiranofaciens ZW3, which has a lot of industrial applications.