Biomimetic trilayered silk-based electrospun scaffolds for regeneration of dura mater
Muhammad Shafiq, Sadia Habib, Hafsah Akhtar, Shama Naz, Mustafa Özgür Öteyaka, Asma Tufail Shah, Fahad Hussain Alhamoudi, Aqif Anwar Chaudhry, Hamad Khalid, Ather Farooq Khan
Abstract
after 48 hours. Furthermore, the fibroblast cell line was exploited to assess the biological properties of TLS. Cell culture results indicated that TLS promoted NIH3T3 proliferation in the 3D microenvironments and was non-toxic. Similarly, live/dead and migration assay results further confirmed the biocompatibility of TLS with increased cell viability and 99% wound closure after 24 hours. Overall, we were able to manufacture a trilayered scaffold that architecturally mimics the native structure of dura mater.
Topics & Concepts
ElectrospinningSILKDura materScaffoldRegeneration (biology)Materials scienceNanofiberBiomedical engineeringComposite materialPolymerAnatomyCell biologyMedicineBiologyElectrospun Nanofibers in Biomedical ApplicationsSilk-based biomaterials and applicationsNerve injury and regeneration