Elizabethkingia argenteiflava sp. nov., isolated from the pod of soybean, Glycine max
Jun-Hee Hwang, Jihyeon Kim, Jae-heon Kim, SangJoon Mo
Abstract
Soybean pods, separated and enclosed from the outside environment, are considered a suitable place to find new microbes. A Gram-stain-negative, aerobic bacterium, bacterial strain (YB22 T ) was isolated from the pod of Glycine max (soybean) collected from a rural area in Republic of Korea and characterized by using polyphasic taxonomy. Cells of the strain were rod-shaped (approximately 0.4–0.6 µm wide and 4.0–5.0 µm long), non-flagellated and formed silver-yellow colonies. Cells grew at 25–35 °C (optimum, 28–30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and with 0–2.0% NaCl (optimum, 0 % NaCl). 16S rRNA gene sequencing showed that strain YB22 T was phylogenetically closest to the genus Elizabethkingia , and showed highest similarities to Elizabethkingia occulta G4070 T (96.7 %), Elizabethkingia meningoseptica ATCC 13253 T (96.7 %), Elizabethkingia miricola DSM 14571 T (96.6 %), Elizabethkingia bruuniana G0146 T (96.5 %), Elizabethkingia ursingii G4122 T (96.4 %) and Elizabethkingia anophelis R26 T (96.2 %). Average amino acid identity values between strain YB22 T and other taxa in the genus Elizabethkingia were all above the threshold range of genus determination. Average nucleotide identity and digital DNA–DNA hybridization values between strain YB22 T and other phylogenetic relatives were all found to be below the threshold range for species determination. The respiratory quinone of strain YB22 T was menaquinone 6 (MK-6) and the predominant cellular fatty acids were iso-C 15 : 0 (47.8 %) and iso-C 17 : 0 3-OH (18.5 %). The major polar lipids were phosphatidylethanolamine, four unidentified aminolipids and three unidentified polar lipids. The phylogenetic analysis and physiological and biochemical data showed that strain YB22 T should represent a novel species in the genus Elizabethkingia , for which the name Elizabethkingia argenteiflava sp. nov. is proposed. The type strain for this novel species is YB22 T (=KCCM 43263 T =JCM 32097 T ).