Rapid Identification of SARS-CoV-2 Variants of Concern Using a Portable <i>peak</i> PCR Platform
Philippe Bechtold, Philipp Wagner, Salome Hosch, Denise Siegrist, Amalia Ruiz-Serrano, Michele Gregorini, Maxmillian Mpina, Florentino Abaga Ondó, Justino Obama, Mitoha Ondo’o Ayekaba, Olivier Engler, Wendelin J. Stark, Claudia Daubenberger, Tobias Schindler
Abstract
PCR. The assays achieved excellent diagnostic performance when tested with RNA extracted from culture-derived SARS-CoV-2 VOC lineages and clinical samples collected in Equatorial Guinea, Central-West Africa. Simplicity of usage and the relatively low cost are advantages that make our approach well suitable for decentralized and rapid testing, especially in resource-limited settings.
Topics & Concepts
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Identification (biology)Computational biology2019-20 coronavirus outbreakSingle-nucleotide polymorphismVirologyGeneBiologyGenotypeGeneticsMedicinePathologyInfectious disease (medical specialty)OutbreakBotanyDiseaseSARS-CoV-2 detection and testingBiosensors and Analytical DetectionSARS-CoV-2 and COVID-19 Research