Design of spontaneously blinking fluorophores for live-cell super-resolution imaging based on quantum-chemical calculations
Ryo Tachibana, Mako Kamiya, Akihiko Morozumi, Yoshiyuki Miyazaki, Hiroyoshi Fujioka, Aika Nanjo, Ryosuke Kojima, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Toshitada Yoshihara, Seiji Tobita, Yasuteru Urano
Abstract
Spontaneously blinking fluorophores are powerful tools for live-cell super-resolution imaging under physiological conditions. Here we show that quantum-chemical calculations can predict key parameters for fluorophore design. We applied this methodology to develop a spontaneously blinking fluorophore with yellow fluorescence for super-resolution imaging of microtubules in living cells.
Topics & Concepts
SuperresolutionResolution (logic)Live cell imagingNanotechnologyHigh resolutionQuantumChemistryCellBiophysicsMaterials sciencePhysicsComputer scienceRemote sensingBiologyComputer visionImage (mathematics)Artificial intelligenceBiochemistryQuantum mechanicsGeologyAdvanced Fluorescence Microscopy TechniquesAdvanced Electron Microscopy Techniques and ApplicationsPhotosynthetic Processes and Mechanisms