Expression of blue pigment synthetase a from Streptomyces lavenduale reveals insights on the effects of refactoring biosynthetic megasynthases for heterologous expression in Escherichia coli.
Tien T. Sword, J. William Barker, Madeline E. Spradley, Yan Chen, Christopher J. Petzold, Constance B. Bailey
Topics & Concepts
BiologyStreptomycesHeterologous expressionEscherichia coliCodon usage biasBiochemistryStart codonPolyketideRibosomal RNARibosomeGeneGeneticsGenomeBacteriaBiosynthesisRNARecombinant DNAMessenger RNAMicrobial Natural Products and BiosynthesisBiochemical and Structural CharacterizationRNA and protein synthesis mechanisms