The N-terminal substrate specificity of the SurE peptide cyclase
Asif Fazal, Jake Wheeler, Michael E. Webb, Ryan F. Seipke
Abstract
, its substrate specificity is poorly understood. To address this issue, an on-resin SurE cyclisation assay was developed and in combination with SNAC thioesters and kinetic measurements was used to define the chemical space of the N-terminal substrate residue.
Topics & Concepts
ChemistryNonribosomal peptidePeptideSubstrate (aquarium)Substrate specificityResidue (chemistry)Terminal (telecommunication)In vivoStereochemistryBiochemistryCombinatorial chemistryEnzymeBiosynthesisComputer scienceBiotechnologyBiologyOceanographyTelecommunicationsGeologyBiochemical and Structural CharacterizationChemical Synthesis and AnalysisAntimicrobial Peptides and Activities