Single AAV-Mediated CRISPR-SaCas9 Inhibits HSV-1 Replication by Editing ICP4 in Trigeminal Ganglion Neurons
Yuxi Chen, Shengyao Zhi, Puping Liang, Qi Zheng, Mengni Liu, Qi Zhao, Jian Ren, Jun Cui, Junjiu Huang, Yizhi Liu, Zhou Songyang
Abstract
in mouse primary TG neuronal cells. SpCas9 and SaCas9 are able to inhibit HSV-1 infection in Vero cells and mouse TG neuronal cultures with high efficiency and good biosafety. AAV1-mediated delivery of SaCas9 shows great potential in treating HSK and inhibiting HSV-1 in TG neurons. Further investigations may be needed to test the inhibition of latent infections, which may result in the development of novel methods for treating viral diseases.
Topics & Concepts
Trigeminal ganglionReplication (statistics)CRISPRGanglionNeuroscienceBiologyVirologyGeneticsGeneSensory systemCRISPR and Genetic EngineeringHerpesvirus Infections and TreatmentsCytomegalovirus and herpesvirus research